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ibhayoloji-lab-izixhobo

I-Lyophilized Bead Forming System

Ubugcisa obuvelayo becandelo lokuxilongwa kwe-IVD kuvavanyo lwe-PCR
I-Lyophilized-Bead

Yintoni i-lyophilized bead?

Ulwazi olusisiseko
I-Lyophilized bead (Lyobeads), eyaziwa ngokuba pcr bead, yiyo ukulungele ukuya amaso pcr. I-Lyobeads zenziwe ngeerejenti okanye ulwelo. I-reagent okanye ulwelo ngokusebenzisa ulwelo oluchanekileyo oluchanekileyo lwe-micro-impompo ukuya kwi-nitrogen engamanzi ukuze zikwazi ngokukhawuleza umkhenkce zibe yimilo efanayo yamaso amancinane aqinileyo kubushushu obufutshane kakhulu kwaye buphantsi kakhulu. Emva koko waqokelela ezi lyobeads zibe lyophilizer freeze dryer yokugcina emkhenkceni.

Itekhnoloji yeLyobeads inokwandisa ukugcinwa kwe-enzyme okanye imisebenzi yeprotheni, ukongeza, amaso reagent lyophilized babe nesakhiwo sothungelwano esikhululekile, banokuphinda banyibilike ngokukhawuleza, kwaye iteknoloji ye-lyobeads inokuguqula ii-reagents zemichiza ezingazinzanga zibe kumgangatho ophezulu, ozinzile, kunye nobuninzi. amaso lyophilized, ekulungele ngokukhethekileyo ukugcina amayeza kunye nokuthutha. Ziya zithandwa ngakumbi itekhnoloji entsha namhlanje.
Ithemba lyophilized bead
Initrogen yolwelo lyophilized bead ziye zaphuhliswa ngokukhawuleza kule minyaka mibini idlulileyo njengenye yetekhnoloji eshushu esakhulayo kwi IVD (Ukuxilongwa kwe-vitro) icandelo loxilongo. Nokuba kuse-United States, eJamani, e-United Kingdom, eRussia, okanye eTshayina, iinkampani ezininzi zaphesheya kweelwandle kunye neenkampani zokuxilonga iimolekyuli zasekhaya zilungiselela uphuhliso, umthamo omncinci, kunye nemveliso eninzi yetekhnoloji entsha. umso pcr.

Kwimiboniso ye-IVD, ukusuka kubakhiqizi be-reagent ukuya kubenzi bezixhobo ze-IVD, ingqalelo kwi-teknoloji ye-lyobeads iyaqhubeka nokunyuka. Njengoko iinkampani zamazwe ngamazwe ikhawulezisa ukukhuthazwa lyobeads iteknoloji kwaye baqalise ubeko imveliso mass. Kungekudala, lyobeads iya kuba yeyona nto iphambili kwicandelo le-IVD.

Ukongeza, thelekisa kwi lyobeads, i-reagents ye-liquid idinga ukuthuthwa kwekhonkco elibandayo kunye nokugcinwa kweqondo lokushisa eliphantsi, elifuna iindleko eziphezulu zokuhamba kunye nokugcinwa, kwaye ukusebenza akuzinzile, umhla wokuphelelwa yisikhathi ufutshane kakhulu. Ngoko ke, i-lyobeads ineenzuzo ezininzi. Ngokuncitshiswa kweendleko zemveliso eziziswa yimveliso yobuninzi kwixesha elizayo, i-lyobeads iya kunceda ukukhula okuqhumayo kwe-IVD yokuxilongwa ngokukhawuleza.

Ukusetyenziswa kweteknoloji ye-lyophilized bead

ukulungele ukuhamba-pcr-amaso
Itekhnoloji yeLyobeads ithetha ukuba i-reagent ye-liquid ikhenkcezwa ngaphambili ibe yi-solid-state yinitrogen engamanzi, ize ikhutshelwe ngamanzi yi-lyophilizer phantsi kwe-vacuum kunye nemekobume yobushushu obuphantsi. Iyakwazi ukwenza umgangatho ophezulu kwaye izinzile amaso lyophilized. Ngoko ke, amashishini amaninzi asebenzisa iteknoloji ye-lyobeads kwimveliso kunye nophando. Kwaye izicelo ezinkulu lyobeads ikakhulu kwiinkalo ezintathu: Ukuxilongwa Biochemical, ukuxilongwa immunologic, kunye nokuxilongwa molekyuli.

Ukuxilongwa kwe-biochemical

lyo-bead
Kwi-biochemical diagnosis, lyophilized bead Itekhnoloji isetyenziswa kwinkqubo yovavanyo eyenziwe ngumhlalutyi we-biochemistry, i-biochemical reagents, i-calibration, ikwabekwe kwilebhu yezonyango esibhedlele, kwiziko lovavanyo lomzimba ukwenzela uvavanyo lwesiqhelo lwe-biochemical. Ukongeza, itekhnoloji ye-lyobeads isetyenziswa ngokubanzi kwisicelo sentengiso yezilwanyana. Njengoko oogqirha bezilwanyana bafuna ukubonwa kwe-PCR, ukuvela kwe-lyobeads kusombulula kakhulu iingxaki zolwelo PCR reagent ukusilela kokuthotywa kwiqondo lobushushu eliqhelekileyo, ukusebenza kakuhle kogcino olufutshane, kunye neendleko eziphezulu zokuthuthwa kwekhonkco elibandayo.

Ukuxilongwa kwe-immunologic

I-Lyobeads ingasetyenziselwa i-chemiluminescence, i-enzyme-linked immunosorbent, igolide ye-colloidal, i-biochemical immunoturbidity, izinto ze-latex, isixhobo esikhethekileyo seprotheni, njalo njalo.
I-chemiluminescence analyzer yimveliso eyenziwe ngama-reagents, izixhobo kunye neendlela zokuhlalutya. Ezifana neprotein yemolekyuli lyophilization kwaye microbial lyophilization ezisetyenziselwa ikakhulu ukuxilongwa kwamathumba, iihomoni, izifo ezosulelayo kunye nezinye izinto.

Ukuxilongwa kweemolekyuli

Oku kubhekiselele ekusetyenzisweni kobuchule bebhayoloji yemolekyuli ukufumanisa iiprotheyini zesakhiwo, ii-enzymes, ii-antigens, izilwa-buhlungu, kunye neemolekyuli ezahlukeneyo ezisebenza kwi-immune ezinxulumene nezifo. Ikwasetyenziswa kwimizila yemfuza ekhowuda ezi molekyuli. Okwangoku, lyobeads in ukuxilongwa kwemolekyuli ziye zasetyenziswa kakhulu ekubhaqweni kweentsholongwane, isifo sofuzo, amathumba, ukuxilongwa kwezilwanyana, ukubhaqwa kokhuseleko lokutya, ubhaqo lweesampulu zokusingqongileyo, kunye nokuzalanisa izityalo.

Izinto ezilungileyo zokusebenzisa i-lyophilized bead

lyophilization-of-proteins
Kukho iingenelo ezininzi zokusebenzisa i-lyophilized bead, ngokubanzi, kukho amanqaku alandelayo: 
1. Ukuchaneka okuphezulu kokufumanisa
2. Iteknoloji entsha yokuxilongwa ngokukhawuleza
3. Inokugcinwa kwaye ihanjiswe kwiqondo lokushisa legumbi emva kwe-encapsulation. Ukuze ikwazi ukunciphisa iindleko zokuhamba.
4. Isakhiwo se-lyobeads yinethiwekhi ekhululekile, inokuphinda idibanise ngokukhawuleza.
5. Ukwandisa umsebenzi we-enzymes / iiprotheni
6. Uluhlu olubanzi lokugubungela isicelo, ukusuka kuvavanyo lwe-nucleic acid, ukubonwa kwe-PCR, kunye nokuxilongwa kwe-immunodetection, ukukhangela kwendalo kunye nokunye.

Inkqubo yokuvelisa i-lyophilized bead

Kukho amanyathelo amahlanu abandakanyekayo kwimveliso ye lyophilized bead:
lyophilisation-nkqubo
Amanqaku aphambili obugcisa ngala alandelayo:
Impompo encinci
-- Luhlobo olutsha lwesixhobo sokumpompa esihambisa izixa ezincinci zolwelo ngokuchanekileyo, ngomzuzu, ngokufanayo, nangendlela eqhubekayo.
-- Ukuchaneka okuphezulu, ukungaguquguquki, kunye nokufana nokunikezela ngezinto ezisebenza ngolwelo.
-- Inani elandisiweyo lamajelo, ukusuka kwizilingo ezincinci ukuya kwimveliso yobuninzi.
-- Ubungakanani bethontsi buyahlengahlengiswa.
Initrogen yolwelo
-- Ukusebenzisa initrogen elulwelo kunokwenza iirejenti ezithe zakhutshwa ngempompo encinci zenza umkhenkce.
-- Inkqubo yokusebenza inokuvumela i-nitrogen engamanzi ukuba yongezwe ngokukhuselekileyo kwaye ngokuzenzekelayo kwi-reagent
lyophilization-iteknoloji
Ukudlulisa
Emva kwenyathelo lenitrogen elulwelo, ezi zixhobo zokubumba ziya kuhanjiswa ngokukhawuleza nangokukhuselekileyo kwisikomisi somiso. Yonke le nkqubo isebenze kakhulu ngokuzenzekela.
Ukwahlula
Kwisixhobo esomisa umkhenkce, esi sixhobo siya kusasaza ngokuzenzekelayo ii-lyobeads kwiifom ezahlukeneyo ngokukhuselekileyo kwaye ngokukhawuleza, emva koko ukupakisha, ukupakisha imveliso epheleleyo.
-- Kukho iindidi ezintathu ezinokuthi zisetyenziswe ukuthwala i-lyobeads, ezi zi-8-linked / PCR / iityhubhu enye, iibhotile zeCeline kunye neediski.
lyophilization-umgubo

Ukusebenza kwe-lyophilized bead

lyo-amasoumso-pcrlyophilized-reagent-amaso

Ubunzima bobugcisa bokubumba i-lyophilized bead

As amaso lyophilized ibe ngakumbi nangakumbi ethandwa, imfuno nitrogen engamanzi lyobeads ukwenza inkqubo iyanda. Ngoko ke, ukusombulula ubunzima bobugcisa bolwelo lwenitrogen lyobeads yeyona mfuneko iphambili yokwenza izixhobo ezikumgangatho ophezulu.
Ubunzima bobugcisa bolwelo lwenitrogen lyobead bukwimiba emithathu:
1. Microdosing liquid
Imiqulu eqhelekileyo yokukhupha isuka kwi-2ul ukuya kwi-100ul. Kunzima ukukhupha ngokuqhubekayo kwaye ngokuqhubekayo kwiimali ezincinci ezinjalo ukuba usebenzisa iimpompo zesirinji eziqhelekileyo okanye iipayipi. I-Lyobeads ifuna ukuchaneka okuphezulu kunye nolwelo lokukhupha. Ngexesha lokusebenza kwibhubhoratri, njengoko ukungahambi kakuhle kwencwadana kunye nezixhobo ezilula, kunzima ukulawula ukuchaneka kunye nokuhambelana. Ngoko ke, kwimeko ye-microdosing liquid, iya kubangela ukuba imilo ye-lyobeads ihluke ngobukhulu ukuba i-micro pump inokulawulwa kakubi kunye nokuchaneka. Oku kuya kuchaphazela ukupakishwa okulandelayo kwitreyi ye-reagent okanye ikhadi, inkqubo yokukhangela, kunye neziphumo zokubona.

2. Yongeza ngokukhuselekileyo ulwelo lwenitrogen
Ngenxa yobushushu obuphantsi kunye nokuguquguquka okuqhubekayo kwenitrogen engamanzi, kuyimfuneko ukuqinisekisa ukhuseleko xa ukongeza ulwelo lwenitrogen, qinisekisa ukungena komoya kakuhle elabhoratri, kwaye rhoqo ukongeza kunye nokongeza ulwelo lwenitrogen.

Kwimeko yokongezwa okwenziweyo kwenitrogen engamanzi, umqhubi kufuneka aqinisekise ukuba isikhongozeli senitrogen engamanzi somile kwaye ujonge ukuba isikhongozeli sibotshiwe na okanye phantsi kwezinye iimeko ezingaqhelekanga. Okwangoku, indlela eqhelekileyo yokuzalisa i-nitrogen yolwelo yindlela yokugalela yefunnel ngokuthe ngqo, le ndlela idinga ukulawulwa komqhubi kwaye ifuna ukumisa rhoqo kumatshini ukongeza initrogen engamanzi. Oku akugcini nje ukwandisa ubuninzi bomsebenzi womqhubi, kwaye kunciphisa kakhulu ukusebenza kakuhle kwemveliso.

3. Ingqokelela lyobeads
Emva kokuba i-lyobeads iveliswe ngokupheleleyo emini, i-lyobeads kufuneka iqokelelwe ngokukhawuleza kwaye ikhuselekile kwizikhongozelo ze-nitrogen zamanzi, kuba ezinye ii-lyobeads azikwazi ukuhlala kwindawo yobushushu obuphantsi.

Izixhobo zokuvelisa ukwenza i-lyophilized bead

1. Umphezulu webhentshi lyobeads ukwenza inkqubo

lyophilized-bead-forming-system
Umsebenzi wokusasaza ochanekileyo omncinci: lo msebenzi uthatha impompo ye-micro-ceramic echanekileyo kunye nomthamo ophantsi njenge-1mg. Izinto eziphathekayo ziyi-ceramics-grade-grade ceramics kunye ne-316L yensimbi engenasici, engaphenduliyo ngolwelo. Umthamo kunye nesantya solwelo olukhutshwayo sinokuhlengahlengiswa. Ukongezelela, zonke iintlobo zeenaliti ezikhethekileyo ziyakwazi ukulungelelanisa, ezi zinokusombulula iingxaki eziqhelekileyo ezijongene nabathengi, ezifana ne-Lyobeads zilula ukuwisa okanye ukugqabhuka, ukuchaneka akuchanekanga, kunye nesiphumo esibi kunye nokuhambelana kwe-beads-forming.

Ulwelo oluzenzekelayo lwesixhobo sokutyisa initrogen: Ngexesha lokusebenza komatshini, isivamvo esiphakamileyo nesisezantsi sibona utshintsho lwenqanaba lolwelo kwisikhongozeli senitrogen ngokuzenzekelayo, kwaye umqondiso ugqithiselwa ngokuzenzekelayo kwisilawuli senqanaba lolwelo, esivula ngokuzenzekelayo okanye sivale ivalve ukuqinisekisa ukuba ulwelo. indawo kwisikhongozeli senitrogen engamanzi kwindawo yokusebenza. Esi sixhobo siphucula imveliso kakhulu.

Isixhobo sokuqokelela i-Lyobeads: Esi sixhobo siya kuqokelela lyobeads ngokukhawuleza ukuba ugcino lyobeads kwisixhobo ifikelela inani elithile okanye reagents ezikhethekileyo ezingenakuhlala ixesha elide nitrogen engamanzi.

Isixhobo sincinci ukwenzela ukuba indawo igcinwe kwaye kulula ukuhamba.
lyobeads-ukwenza-inkqubo

2. Umgangatho wokumisa lyobeads inkqubo yokwenza

lyophilizer-freeze-dryer
-- Ukuchaneka okuphezulu, okuphantsi kungaphantsi kwe-1%, ukuphindaphinda okuphezulu, kunye nokuthembeka.
-- Isantya sokusebenza sikhawuleza kakhulu, imveliso ephezulu.
-- Ukuzenzekela. Le nkqubo inokuqhutywa ngokuzenzekelayo. Inomsebenzi wokhetho lohlengahlengiso lwedijithali lomthamo we lyobeads.
-- Iitshaneli ezininzi azikhethi
-- uyilo lobuntu, kukulungele ukuqokelela lyobeads
-Ii-Reagents zinokugcinwa kwiqondo lokushisa eliphantsi kunye nobushushu obuqhubekayo kunye nokuphepha ukukhanya.
-- Ukusebenzisa insimbi engenasici ukwenzela ukuba ingaphenduli kunye ne-reagents.
-- Yongeza umsebenzi wokuvuselela i-reagent ukugcina i-reagent ngokulinganayo.
-- Umthamo omkhulu unokuvelisa umthamo
-- Isantya: ubuninzi 9,000 amaso ngeyure

3. Umatshini wokupakisha we-Lyophilized (ozenzekelayo ngokupheleleyo)

lyophilization-oomatshini
Ukupakisha ngokuzeleyo i-lyophilized bead, amaso angama-2,500 (i-8 strips pcr tube) ngeyure
lyophilized_bead

Indlela yokwenza lyobeads inkqubo?

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