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Cell Counter

Circulating water bath used in laboratory

1. What is cell counter?
2. Methods used for cell counting
3. Automated cell counter
    3.1 Use of cell counter
    3.2 Application of cell counter in experiments
    3.3 Application of cell counter in experiments
4. How to buy cell counter?

What is cell counter?

To ensure the quality of the cells for subsequent experiments, many laboratories test the concentration and viability of the cells, traditionally by means of Taipan blue dye for mammalian cells and melanin dye for yeast. Both dyes stain the nucleus, and when the cells are alive, the membrane is selectively permeable and the dye cannot enter the cell. When the cell is dead, the selective permeability of the membrane is lost and the dye can enter the nucleus and stain the cell. This allows the cells to be distinguished as dead or alive. Traditional blood counters with the complex operation and large errors have been increasingly replaced by automated cell counters. Cell counter has many features that ensure the accuracy and stability of the count results.

a. Accurate and fast basic counting function. Apply multiple technologies such as high resolution, automatic optical focus, multi-field analysis and high statistical sampling volume to ensure the accuracy and reproducibility of counting results.

b. Innovative agglomerated cell correction function. For ordinary cells and agglomerated cells, different modes are preset, and the innovative agglomerated cell correction function is available for agglomerated cells.

c. Rigorous manual correction of impurities function. The result deviation caused by impurity foreign matter can be removed manually on the basis of software analysis.

d. One-stop data statistical analysis function. It has a cell proliferation curve (CTC), cell diameter distribution, cell nodule distribution, and superimposed analysis of each statistical graph.

e. Flexible and diverse data output functions.

f. Advanced data security protection features. Equipped with a software operation security key, the software system sets user login privileges and stores all experiment results automatically.

Methods used for cell counting

Cell counter machine

A. Taipan blue staining

The staining principle is that Tissue blue cannot penetrate the intact cell membrane of living cells, so living cells are not colored; whereas the cell membrane of dead cells is damaged and the dye is enriched through the cell membrane to make the cells blue. However, Tissue blue staining does not always accurately distinguish between nucleated cells and cellular debris, so it tends to underestimate the total number of cells and overestimate cell viability.

B. Fluorescent cell counting

Acridine orange/propidium iodide (AO/PI) staining principle: Live cells fluoresce green with AO staining, while dead cells fluoresce red with PI staining. Importantly, cells with complex backgrounds, such as peripheral blood mononuclear cells, containing a high number of cellular debris or cells in clusters are counted, avoiding the detection of cellular debris. This approach has also recently been applied to single-cell genomics applications, where green corresponds to intact cells and red corresponds to successfully isolated nuclei. This strategy allows researchers to calculate the percentage of unlysed residual cells to the total cell count, while counting nuclei, suggesting sample quality and helping researchers determine whether to proceed with single-cell genomics processes.

C. Automated fluorescence cell counter

Fully automated fluorescence cell analyzer, the combination of bright field + dual color fluorescence channel makes the experiment easier and faster, and also has a transfection detection function, which widens the width of detection even more. The detection of a larger variety of cells is not only applicable to simple cultured cells, but also allows rapid counting and analysis of cell specimens of widely different types and origins isolated from tissues, bone marrow, peripheral blood, alveoli, and liver. The fluorescent digital images obtained by microscopy are automatically analyzed by fluorescent labeling technology and image classification recognition technology to obtain the dead or alive status of cells, and apoptosis and to realize the classification of cells.

Technical parameters

a. Various detection modes: non-staining counting + staining counting + two-color fluorescence counting + manual calibration mode + transfection.

b. Auto-focus, no manual intervention for focus adjustment.

c. Fully automatic carrier table: automatic movement in X and Y directions can be realized; conforming to microscope counting standards, 4 standard area areas can be collected automatically.

d. Pixel: 10 million color camera.

e. Detection of cell diameter range: 2-200μm.

f. Measuring cell concentration range: 0.25x104- 3×107 cells/mL.

g. Compatible with reusable standard glass counting plates.

h. Image acquisition: click the counting key, and the instrument automatically takes 4 area images, automatically completes the acquisition, storage functions, identification, statistics, report generation, etc.; images can be multi-channel superposition, and image brightness can be adjusted.

i. With double-well counting mode: it can automatically obtain the images of cells in two sample slots on the same counting sheet, and the software automatically calculates the average value for more accurate results.

j. Pattern recognition functions: cell fragment exclusion analysis; individual cell counting of clustered cells; irregular cell counting; data analysis charts provided including but not limited to: cell diameter analysis; cell diameter mean analysis; cell size histogram; cell diameter-brightness scatter plot; cell diameter-brightness contour plot; growth curve chart; cluster distribution chart; four regions cell original chart; four regions cell dead or alive identification chart; four regions stitching chart; provide puzzle viewing tool, all count history data can be retrieved at any time to view and trace.

Automated cell counter


Use of cell counter

Cell counting is basic research in cell culture research. It is an important means to understand the growth state of cultured cells and determine the biological effects of media, serum, and drugs. The automated cell counter can analyze cultured cells in less than 1min and obtain cell concentration, cell means volume size, viability, cell size, or volume distribution curves. Automatic cell counters are also used for algae, microorganisms, and other organisms in the range of diameter cell counters. Compared with the blood cell counting plate method, the cell counting machine is more accurate, fast and convenient, and the error is smaller.

Application of cell counter in experiments

The concentration of cells in the culture medium at different time periods was measured, and the cell growth curve was drawn to determine the doubling time of cells. This is a necessary part of the cell culture process. The metabolic pathways in cells are different during different periods of growth.

a. Cell counting. When measuring the intra- and extracellular metabolites, it is possible to know the intra- and extracellular metabolites of each cell, avoiding errors due to different cell counts in parallel or control experiments.

b. Cell viability. Cell viability can be used in experiments such as cytotoxicity experiments, drug treatment experiments, and apoptosis experiments.

c. Obtaining cell volume histograms can be obtained to distinguish the morphology of cells.

Advantages of cell counter

A. Extremely convenient operation

Cell counter is easy to operate and only requires three simple steps to complete the cell concentration viability determination.

a. Load sample

b. Insert counter board

c. Get the result

Users only need to sample 20 microliters, insert the mounting plate into the instrument, and click start within 20 seconds to get the concentration and viability of cells.

B. No manual focus is required

All counters based on image analysis need to get clear cell images at the correct focal length, and then perform image analysis to get counting results. If the focal length is not adjusted correctly, it will seriously affect the concentration and the judgment of survival. If the focal length is not properly adjusted, it will seriously affect the judgment of life and death, and affect the stability of the counting results. In the process of using the cell counter, there is no need to manually focus, which greatly avoids the impact of focal length error on the determination of life and death.

C. Good depth of field performance

When the lens is in sharp focus on an object, the points on the same plane perpendicular to the axis of the lens in the center of the lens can be quite clear on the film or receiver, and the points in a certain range along the front and back of the lens axis in this plane can also form relatively clear image points acceptable to the eye. The distance between all objects in front of and behind the plane is called the camera's depth of field.

The depth of field for the cell count is the depth of the cell suspension that can be clearly imaged on the CCD. A typical counter board has a depth of 100-200 microns, and if the depth of field performance of the lens is not good, some cells within the depth of field will not be able to image. The ideal counter should be able to visualize the entire depth of the plate. The scene is deeply affected by the focal length, object distance, aperture size, and CCD size. Weighing various factors, the cell counter designed an imaging system with a large depth of field to ensure that the cells in the counting plate could be clearly photographed and that the stability and accuracy of the counting results were guaranteed.

How to buy cell counter?

ANTITECK provide lab equipment, lab consumable, manufacturing equipment in life sciences sector.
If you are interested in our cell counter or have any questions, please write an e-mail to [email protected], we will reply to you as soon as possible.

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