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Lab Disinfectant

Lab disinfectant used in laboratory

What is lab disinfectant?

Lab disinfectant is a surface-active cleaning solution, and radioactive contamination decontamination agents can be used in the laboratory, medical and special industrial sectors. It is biodegradable, phosphate-free, highly bactericidal, rinseable, and non-flammable, and available as a concentrated liquid that can be diluted with water.

Disinfectant used in laboratory is an alkaline emulsion concentrate (pH 13+) composed of an amphoteric surface activator, stabilizer, alkali, non-phosphate detergent, and multivalent chelator.

Lab disinfectant is used for cleaning or contamination decontamination of glass products, ceramic products, plastic products (non-polycarbonate), rubber products, stainless steel products, and ferrous metal products*, safe and effective. Laboratory cleaners as alkaline substances, not suitable for non-ferrous metal products, especially aluminum and zinc products, not suitable for polycarbonate products, and not for home use.

Types of lab disinfectants and configuration methods

In chemical experimental work, washing instruments is not only a must-do pre-laboratory preparation work but also technical work. The cleanliness of laboratory instruments directly affects the experimental effect, and even determines the success or failure of the experiment. Due to the unclean or contaminated vessels, often resulting in large experimental errors, and even the opposite of the experimental results. Therefore, for schools, research institutes, hospitals and factories, and mining enterprises of each laboratory, the washing of instruments are very important.

General lab disinfectant

Disinfectant used in laboratory commonly contains soap, soap solution, laundry detergent, detergent powder, washing-up liquid, etc.

a. Soap, soap solution, laundry detergent, detergent powder, and other laboratory cleaning agents are used for instruments that can be scrubbed directly with a brush, such as beakers, triangular flasks, reagent bottles, etc.

b. Lab disinfectant can be used for instruments that are not easy to wash with a brush, such as burettes, pipettes, volumetric flasks, distillers, and other specially shaped instruments, as well as for washing long-unused cups and utensils, and scales that cannot be brushed.

Notes on the preparation and use of lab disinfectant

There are many types of disinfectants used in the laboratory, the common ones are strong acid oxidant wash, alkaline wash, pure acid, pure alkali wash, organic solvent wash, decontamination solution, etc.

Strong acid oxidant detergent can effectively remove oil, handprints, and other dirt; alkaline detergent can effectively remove the dirt on the inner wall of glassware, but its erosion of glassware is strong, so attention should be paid to controlling the cleaning time, and should not be too long; pure acid solution such as industrial concentrated hydrochloric acid can remove some inorganic salt precipitation and scale; decontamination solution can destroy aflatoxin, benzopyrene, etc.
A. Strong acid oxidizer wash
The strong acid oxidizer wash is made of dichromate A (K2Cr2O7) and concentrated sulfuric acid (H2SO4), K2Cr2O7 has a strong oxidizing ability in acidic solutions, and has little erosion effect on glass instruments. Therefore, this wash is widely used in laboratories*. Chromium is carcinogenic, so the preparation and use of the wash should be extremely careful, the two commonly used preparation methods are as follows.

a. Prepare a 12% solution of 500 mL. Take 60 g of industrial K2Cr2O7 in 100 mL of water (the amount of water added is not fixed, to be able to dissolve), heat to dissolve, cool, slowly add 340 mL of concentrated sulfuric acid, stirring while adding, and then store in a glass jar with a ground mouth after cooling.
b. Weigh 5g of potassium dichromate powder, put it in a 250mL beaker, add 5mL of water to dissolve it, then slowly add 100mL of concentrated sulfuric acid, the temperature of the solution will reach 80℃, wait for it to cool down and store it in a glass bottle with a polished mouth.
The wash is a strong oxidizing agent, but the oxidizing effect is relatively slow, direct contact with the utensils for several minutes to several hours to have an effect, after removal to fully rinse with tap water 7 times -10 times, and finally 3 times with pure water.

This wash should be used with care not to splash on the body, to prevent "burning" broken clothes and damage to the skin. When pouring the lotion into the instrument to be washed, make sure that the entire wall of the instrument is dipped, and then pour it back into the lotion bottle after a short pause. After rinsing the instrument with a small amount of water for the first time, the wastewater should not be poured into the sink and sewer, as it will corrode the sink and sewer for a long time, and should be poured into the waste tank and discarded after special treatment.
B. Alkaline lotions
Commonly used alkaline solutions in the laboratory include sodium carbonate (Na2CO3, i.e. soda ash), sodium bicarbonate (Na2HCO3, baking soda), sodium phosphate (Na3PO4, trisodium phosphate), disodium hydrogen phosphate (Na2HPO4), alkaline potassium permanganate solution, etc.

Alkaline potassium permanganate solution preparation method: take 4 grams of potassium permanganate (KMnO4) and dissolve it with a small amount of water, then add 10% sodium hydroxide (NaOH) 100mL, mix well and prepare for use.
Alkaline washing solution is used to wash instruments with oil and dirt, with this washing solution is used long time (more than 24 hours) soaking method or immersion boiling method. When retrieving instruments from the alkaline washing solution, wear latex gloves to avoid burning the skin.
C. Pure acid and pure alkali washing solution
According to the nature of the dish dirt, directly use concentrated sulfuric acid (HCL) or concentrated sulfuric acid (H2SO4), or concentrated nitric acid (HNO3) to soak or boil the dishes (the temperature should not be too high, otherwise the concentrated acid volatilization stimulates people).

Soda ash washing solution with more than 10% of concentrated caustic soda (NaOH), potassium hydroxide (KOH), or sodium carbonate (Na2CO3) liquid soaking or boiling vessels (can be boiled).
D. Organic solvent washing solution
For vessels with fatty dirt, they can be scrubbed or soaked with organic solvents such as gasoline, acetone, toluene, xylene, alcohol, trichloromethane, and ethyl (yttrium) ether. Among them, toluene, xylene, gasoline, etc. can wash the grease, alcohol, ethyl (yttrium) ether, acetone can rinse the instruments just washed with water.

Organic solvents are wasted as washing solutions, so we try to use alkaline washing solutions for large instruments that can be scrubbed with a brush. Only small pieces or specially shaped instruments that cannot be washed with a brush are washed with organic solvents, such as piston bore, pipette tip, burette tip, burette piston hole, burette, vial, etc.
E. Decontamination solution
To prevent harm to the human body, the utensils for testing carcinogenic chemical substances should be soaked with a decontamination solution that has a destructive effect on these carcinogenic substances before washing. The decontamination solutions often used in the test are 1% NaOCL solution, 5% NaOCL solution, 20% HNO3 solution, and 2% KMnO4 solution. Soak the contaminated glass instruments with 1% NaCl solution for half a day or soak them with 5% NaCl solution for a few moments, you can achieve the destruction of aflatoxin.

NaOCL solution preparation method is as follows.

a. Take 100 grams of bleaching powder, add 500 mL of water, stir well, and dissolve 80 grams of industrial Na2CO3 in 500 mL of warm water, then mix the two liquids, stir, clarify and filter, the filtrate containing 2.5% of NaOCL.

b. Take 100g of bleaching essence, add 500mL of water, stir well, and dissolve 160g of industrial Na2CO3 in 500mL of warm water, then mix the two solutions, stir, clarify and filter, this filtrate contains 5% of NaOCL.

c. If 1% NaOCL solution is needed, the above solution can be diluted in proportion.

d. 20% HNO3 solution has a destructive effect on benzo(a)pyrene. Glass instruments contaminated by benzo(a)pyrene can be soaked in 20% HNO3 for 24 hours, and then washed with tap water to remove the residual acid after removal. Latex gloves and micro-syringes contaminated with benzo(a)pyrene can be soaked in 2% KMnO4 solution for 2 hours and then washed.

Use of lab disinfectant

a. The laboratory cleaner is a concentrated solution. It needs to be diluted with water first before use. The recommended dilution concentration is 2~5%, serious stains can be adjusted upward to use the concentration.

b. When using, soak the utensils in the soaking solution for 2~24 hours, the soaking time depends on the stubborn nature and degree of stains.

c. Heating soaking solution (40~50°c) or using ultrasonic cleaning can greatly shorten the cleaning time.

d. It is recommended that the soaked utensils be rinsed repeatedly as soon as possible using running tap water for more than 3 times, and then rinsed with deionized water or distilled water more than 3 times to ensure thorough removal of stains and cleaning agents.

e. The soaking solution can be repeated and replenished for use. If the soaking solution is too dirty, or the PH value is below 8, it can be regarded as unqualified, and appropriate amount of organic acid should be added to neutralize and discharge as appropriate.

f. Please use covered HDPE plastic or stainless-steel containers for immersion.

g. After the utensils are removed from the soaking solution, they should be rinsed immediately and not left for too long before washing.

How to buy lab disinfectant?

ANTITECK provide lab equipment, lab consumable, manufacturing equipment in life sciences sector.
If you are interested in our lab disinfectant or have any questions, please write an e-mail to, we will reply to you as soon as possible.

    AntiTeck Life Sciences Limited

    A1-519, XingGang GuoJi, Yingbin Road, Huadu, Guangzhou, China, 510810
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