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Pasteur Pipette

Pasteur pipette used in laboratory

1. What is Pasteur pipette?
    1.1 Feature of Pasteur pipette
    1.2 Application of Pasteur pipette
2. Type of Pasteur pipette
    2.1 Glass Pasteur pipette
    2.2 Plastic Pasteur pipette
    2.3 Disposable Pasteur pipette
3. Use of Pasteur pipette
    3.1 Use of glass Pasteur pipette
    3.2 Use of plastic Pasteur pipette
4. How to buy Pasteur pipette?

What is Pasteur pipette?

Pasteur pipette, an alias transfer tube, is a kind of laboratory supply made of polyethylene, a transparent polymer material. Pasteurized pipettes are usually classified as gamma-sterilized or non-sterilized. They are mainly used in operations such as aspirating, transferring, or carrying small amounts of liquids for cellular assays, clinical trials, cloning tests, etc.

Feature of Pasteur pipette

a. The tube body has a hollow capsule, which can facilitate the mixing of solvents and agents, cell bodies, etc.

b. The tube body is translucent and bright white, with the ideal liquid flow and easy control.

c. Pasteur pipette can be used in a liquid nitrogen environment.

d. Thin, flexible and bendable body for easy access to micro or special containers

e. Small tip for repeatability of drops.

f. The heat-sealable end for easy transport of liquids.

g. Pasteur pipettes are widely used in genetics, medicine, epidemic prevention, clinical, genetic, biochemical, petrochemical, military, and other fields, and are disposable consumable items in laboratories.

Application of Pasteur pipette

A Pasteur pipette is a piece of laboratory equipment that allows the user to measure and dispense the desired amount of liquid material. Pasteur pipettes are commonly used in laboratory settings when conducting experiments. Scientific disciplines as diverse as chemistry, biotechnology, medicine, and natural sciences use barrel pipettes when performing laboratory work. Graduated Pasteur pipette can measure liquid transfers with +/- 10% accuracy.

Type of Pasteur pipette


Glass Pasteur pipette

The glass Pasteur pipette is a pipette made of a biocompatible material that is resistant to most solvents and cleaning agents, as well as acids and bases. Therefore, it is a high-quality and practical alternative to a plastic Pasteur pipette.

Material of glass Pasteur pipette

a. Soda-lime glass

b. Borosilicate Glass

c. Neutral Glass

Feature of glass Pasteur pipette

Strong alkaline or acidic cleaners are often used in the cleaning process. Unlike the usual plastic Pasteur pipettes, glass Pasteur pipettes are resistant to most solvents and cleaning agents, as well as acids and bases. In addition to this feature, glass Pasteur pipettes are stable during steam sterilization, especially when they are exposed to pressure and high temperatures.

Plastic Pasteur pipettes containing non-liquid samples that evaporate at 100°C may lose their shape stability, soften and deform. In a typical steam sterilization process, pipettes are typically exposed to temperatures up to 134°C, and hot air sterilization is performed at even higher temperatures up to 180°C. Given that glass Pasteur pipettes are thermally stable and can withstand more heat overall than plastic pipettes, they can be sterilized without problems. My glass Pasteur pipettes can even be used for pyrolytic cleaning and may be temporarily exposed to temperatures of up to 500°C.

In addition, the glass does not react with organic substances, such as somatic or germ cells. This is an advantage, especially in the field of in vitro fertilization. Glass also remains stable at high temperatures in smaller structures.
a. Resistant to most solvents and cleaning agents as well as acids and bases.

b. Mechanically stable.

c. The slow rise of the liquid (not continuous straight pipette tip).

d. Suitable for pipetting by mouth (filters available).

e. Suitable for heat, steam, moisture, EO (ethylene oxide), plasma (gas mixtures), and autoclaving.

f. Suitable for gamma sterilization (depending on glass type).
a. Laboratory

b. Chemistry

c. Biotechnology

d. Pharmaceuticals
Practical applications
a. Droppers

b. Microdistillation

c. Pipetting

d. Dosing

e. Serum transfer

f. Aspiration of fluids

g. In Vitro Fertilization

Plastic Pasteur pipette

Plastic Pasteur pipette is usually made of high-clarity polyethylene or high permeability PE. The tube is smooth to the touch, transparent and graduated for direct observation of liquid aspiration. The upper part of the tube has an oblong capsule to mix or aspirate the solvent, agent, cell body, liquid specimen, etc. to be transferred. The smooth wall of the tube facilitates the flow of liquid and is easy to control. The overall slim and soft body of the tube can be bent and quickly rebounded to facilitate access to microvolume or specially shaped containers. The tip is abruptly smaller than the body of the tube to ensure the repeatability of the drop. Its main specifications are 1ml, 3ml, 5ml, 10ml, etc.

Plastic Pasteur pipettes sometimes referred to as "transfer pipettes,", are widely used in the healthcare, laboratory, and healthcare industries. They have been developed for the precise transfer of liquid materials, chemicals, and solutions from one container to another, and they are inexpensive and single-use. Plastic Pasteur pipettes are made from cost-effective raw materials such as polypropylene, which is inert to most acids and biological fluids and was developed specifically for chemical laboratories and molecular biology to provide contamination-free transfer of small volumes of solutions. Plastic Pasteur pipettes can be used in general laboratory equipment, bacteriology, hematology, and blood banks because of the precise safety standards used to produce them.

Disposable Pasteur pipette

Disposable Pasteur pipette, made of selected medical grade LDPE raw material, is suitable for a small amount of aspiration and transfer. It has an optimized surface tension treatment, with a strong liquid flow and easier handling.

Feature of disposable Pasteur pipette

a. Good transparency with graduated lines for easy observation.

b. With a certain elasticity, it can be bent within a certain angle, which is conducive to entering the micro cargo anisotropic container for operations such as taking or adding liquid.

c. Good elasticity, not easy to break, suitable for rapid pipetting.

d. Easy to use, accurate and reliable, with good repeatability of drop volume.

e. The end of the tube can be heat-sealed for the delivery of small amounts of liquid.

f. Lightweight and compact easy to use, low loss of consumables, disposable also reduces the contamination of raw materials in use.

Use of Pasteur pipette

Before using the Pasteur pipette, you need to confirm which liquid the pipette will be used for. This way, you know what to do when handling any liquid material and avoid using the wrong Pasteur pipette.

Use of glass Pasteur pipette

a. A bulb is attached to the top while inserting the bulb no deeper than the top section of the Pasteur pipette. Either a latex or rubber bulb can be used.

b. Squeeze the bulb and insert the tip of the Pasteur pipette into the liquid you wish to transfer. Take care to ensure that the pipette is in an upright position.

c. Gently loosen the bulb and draw the liquid into the Pasteur pipette. Do not draw liquid into the bulb or the top section of the pipette. If you notice liquid squirting out of the bottom of the pipette. Pull in, then expel the liquid several times.

d. Move the Pasteur pipette to the container you wish to transfer the liquid to and gently squeeze the bulb. Again, be sure to keep the pipette upright at all times to avoid liquid entering the bulb.

Reusable glass Pasteur pipette

The reusable conditions of the glass Pasteur pipette are as follows.

a. When you use the glass Pasteur pipette only for solvents. Dry the pipette thoroughly after use and it is safe to use it next time.

b. When you are transferring the same liquid. Keep the glass Pasteur pipette clean between uses by storing it upright in clean glassware (e.g. beaker or Erlenmeyer flask) to keep it clean between uses.

c. If a pipette is broken or can no longer be used for any other reason, rinse all chemicals from it (dispose of the rinse in an appropriate waste container) and then dispose of the pipette in a glass waste.

Use of plastic Pasteur pipette

a. Before use, a cotton plug is inserted into the thick end of a Pasteur pipette and then sterilized. Pipettes blocked by this method are protected from microbial contamination of the transferred liquid. In microbiological studies, graduated Pasteur pipettes, for the same reason, should also be plugged with cotton plugs.

b. When using a plastic Pasteur pipette, pinch the top with your fingers to drive out the air in the dropper, then stick the dropper into the reagent bottle, release your fingers, and the reagent will be inhaled.

c. When adding liquid drops, the Pasteur pipette should be suspended in the air above the beaker.

d. Do not touch the beaker wall to avoid staining the dropper or causing contamination of reagents.

e. It is strictly forbidden to use an unwashed dropper to absorb other reagents.

How to buy Pasteur pipette?

ANTITECK provide lab equipment, lab consumable, manufacturing equipment in life sciences sector.
If you are interested in our Pasteur pipette or have any questions, please write an e-mail to, we will reply to you as soon as possible.

    AntiTeck Life Sciences Limited

    A1-519, XingGang GuoJi, Yingbin Road, Huadu, Guangzhou, China, 510810
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