Cell Culture Dish

Content
1. What is cell culture dish?
    1.1 Specification of cell culture dish
2. Type of cell culture dish
3. Use of cell culture dish
4. Application of cell culture dish
    4.1 Surface treatment technology with culture plates
    4.2 Cell culture dish in microbiology
5. How to buy cell culture dish?

Cell culture dish is a laboratory vessel used for microbial or cell culture, consisting of a flat disc-like bottom and a lid, generally made of glass or plastic. Petri dish materials are divided into two categories, mainly plastic and glass, glass can be used for plant material, microbial culture and animal cell wall culture may also be used. The plastic ones may be made of polyethylene, and there are disposable and multi-use ones, which are suitable for laboratory operations of inoculation, delineation, and separation of bacteria, and can be used for the culture of plant materials.

It was originally designed in 1887 by Julius Richard Petri (1852-1921), a bacteriologist working under the German biologist Robert Koch, and is therefore also known as the "Petri dish". Petri dishes are fragile and fragile, so they should be cleaned and placed with care and caution. It is best to clean the dishes after use and store them in a safe and fixed location to prevent damage and breakage.

Cell culture dish is suitable for epidemic prevention stations, hospitals, biological products, the food industry, the pharmaceutical industry, and other units for the isolation and culture of bacteria, antimicrobial potency test, and qualitative test analysis. In agriculture, aquatic and other scientific research is used for the artificial culture of seeds hair teeth, plants, insects, fish species, and hatching research. Electronic industry or other industries used as vessels.

a. According to the different uses of Petri dishes can be divided into cell culture dishes and bacterial culture dishes.

b. According to the different manufacturing materials, cell culture dishes are divided into plastic culture dishes and glass culture dishes, but the imported culture dishes and disposable culture dishes are plastic materials.

c. According to the size, cell culture dish can be divided into 35mm, 60mm, 90mm and 150mm diameter dishes.

d. According to the different separations, the cell culture dish can be divided into a 2-divided culture dish, a 3-divided culture dish, etc.

e. Cell culture dishes are divided into two categories in terms of material, plastic, and glass. The glass bottom culture dishes can be used for plant material, microbial culture and animal cell applanation culture, etc. Plastic cell culture dishes are mostly made of polyethylene and are divided into disposable and multi-use ones. They are suitable for laboratory operations of inoculation, delineation, and separation of bacteria, and can also be used for the culture of plant materials.

a. Cell culture dish needs to be cleaned and disinfected before use because the cleanliness or otherwise of the cell culture dishes has a greater impact on the work, which can affect the pH of the culture medium, and if certain chemicals are present, they can inhibit bacterial growth.

b. Newly purchased cell culture dishes should be rinsed with hot water first, then placed in a solution of 1% or 2% mass fraction of hydrochloric acid for several hours to remove the free alkaline material, and then rinsed twice with distilled water.

c. If you want to culture bacteria, you need to put a cell culture dish with high-pressure steam (generally 6.8 * 10 of 5 times Pa high-pressure steam), 30min sterilization at a temperature of 120 ℃, placed at room temperature to dry, or with dry heat sterilization, that is, the petri dish is placed in the oven, the temperature control in the case of about 120 ℃ to maintain 2h, you can kill the bacterial cell buds.

d. Only sterilized cell culture dishes can be used for inoculation culture.

When the cell culture dish produces more water vapor, the water vapor will condense on the lid and produce water drops. If the petri dish is square, the water drops will scatter the colonies, so a large colony may be scattered into many small colonies, which will cause great trouble to the culture and counting of bacteria. Therefore, with the medium on top and the lid on the bottom, the water drops will not drip onto the colonies.

Treatment of culture plates with denatured collagen improves the growth of a variety of cells, such as the ability of epithelial cells to adhere. Denatured collagen is covered by pouring a solution of self-extracted collagen or commercially sold (e.g., Akamu, sigma, Thermo Fisher, etc.) substitutes into the culture dish, then simply aspirating the excess solution and allowing the residue to dry.

Collagen can also be used as a non-denaturing gel that supports synaptic growth in chick embryonic spinal ganglia, growth and morphological differentiation of mammary cells, stem cells, liver cells, and other epithelial cells, and promotes the expression of many other tissue-specific functions in isolated cells, commonly used in three-dimensional (3D) cell culture techniques. The collagen is diluted with a medium at a ratio of 1:10. The amount of collagen used can be tested by gradient experiments for the optimum concentration for different cells, and then the pH is adjusted to 7.4. The liquid collagen becomes a gel, so dilution and dispensing must be rapid. Preferably, after the growth medium is added to the gel, it is left to stand at 4°C for 4-24 h to ensure that the gel wants to form an equilibrium with the medium before adding the cells. During this time, additional proteins such as fibronectin or laminin may also be added.

Commercial matrixes such as Becton Dickinson's matrix gels generally contain laminin, fibronectin, and proteoglycan, with laminin being the most abundant. Other matrix products include laminin F, haptoglobin, acetyl heparin sulfate, EHS Natrix, ECL, and Cell-tak. The chemical composition of some of these products is not complete determination, but has been purified; others are mixtures of substrates that are difficult to characterize and may also contain some binding growth factors. These matrices can be used if cell adhesion is important for survival and if there is no need to specify the composition of these adhesions, as in the case of some commonly used tumor cell lines in culture. However, in the case of studying mechanistic issues, these matrices are often used only as an intermediate stage on the path to using adhesions with fully defined composition. Therefore, the use of samples with well-defined composition and high purity, such as gelatin and collagen, as substrates for three-dimensional (3D) cell culture to study mechanisms is becoming increasingly widespread.

If a non-active surface covering is required, appropriate types of monolayer cell cultures may be considered to provide a suitable matrix for some specific types of cells.

Substrate coverings aid in cell attachment, growth, and differentiation. Some difficult-to-culture cells, especially those that are more difficult to culture at low cell densities, require live cells to maintain them. Examples are the culture of multifunctional stem cells (iPS) and NK cells. This may be partly due to metabolite leakage from feeder layer cells or secreted growth factors that have a complementary effect on the culture medium, but also due to cell products that optimize the cell attachment surface.

Sometimes, operators do not want cell attachment when culturing cells because untransformed cells in these adherent matrices do not readily form colonies. For example, when culturing hematopoietic stem cells for colony differentiation, we usually inoculate the hematopoietic stem cells onto an agar gel and supplement it with the relevant cytokines.

This selection system of non-adhesive attachment surface involves two important factors.

a. Prevention of attachment to the bottom of the cell culture dish, because once cells are attached to the bottom, spreading and apposition-dependent growth occurs.

b. Sequestering cells so that daughter cells produced by division can aggregate (even if not adhere) together to form colonies. Agar, agarose, or methylcellulose are currently the most commonly used. The first two are gels and the latter is a highly viscous sol. Because methylcellulose is a solute in which cells can slowly sink, a layer of collagen is often placed beneath it.

Petri dishes are widely used in biology to cultivate microorganisms such as bacteria, yeasts, and molds. It is best suited for organisms that thrive on solid or semi-solid surfaces.

The cell culture medium is usually an agar plate with layers of several millimeters of thick agar or agarose gel containing any nutrients the organism needs (such as blood, salt, carbohydrates, amino acids) and other desired ingredients (such as dyes, indicators, and drugs). Dissolve the agar and other ingredients in warm water and pour into a tray to cool. Once the medium has solidified, inoculate ("plating") the organism samples.

Cell culture dishes are then left undisturbed for hours or days during the growth of the organism and may be placed in an incubator. They are usually covered or placed upside down to reduce the risk of airborne spore contamination.

A virus or phage culture requires that a batch of bacteria be cultured in a Petri dish first, and then the Petri dish becomes the medium for the viral inoculum.

Although Petri dishes are common in microbiological research, smaller Petri dishes are often used for large-scale studies in which culturing cells in Petri dishes can be relatively expensive and labor-intensive.

If you are interested in our cell culture dish or have any questions, please write an e-mail to info@antiteck.com, we will reply to you as soon as possible.