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Sterile Sample Bag

Sterile sample bag used in laboratory

What is sterile sample bag?

sterile-bags-for-microbiology
Sterile sample bag is a safe and non-contaminating sampling container. It is mainly used for the collection, storage and transportation of liquid and solid samples. It is suitable for environmental sampling, biomedical, public health, quality inspection (QA/QC) and food and beverage fields.

Sampling bag sterile

Advantages of sterile sample bag

a. Made of high-strength material, which will not be broken even in the strong beat of a homogenizer.

b. Product is irradiated and sterilized, and the packaging is hygienic and safe.

c. Self-sealing strip at the top of the bag ensures that the inside of the bag is sterile before use.

d. Large bag opening for easy sample addition.

e. Designed as a standing bag, which can stand on its own without the homogenization bag stand after adding samples, saving operation space and facilitating the processing of large batch sampling operations, while not tipping over and causing sample liquid to overflow, thus contaminating the operation table and incubator.

f. Bag body with writing area, convenient for recording.

Features of sterile sample bag

a. It can guarantee the sterility of the sample bag after 5 years of storage

b. Operating temperature range:-100℃ to +80℃. It can be placed in the water bath to preheat the sample or freeze the sample in a freezing environment.

c. 100% aseptic gamma ray and forming process 220-240℃ high-temperature aseptic treatment.

d. The material is LDPE (low-density polyethylene), resistant to UV light, radiation, and various detergents.

e. The sampling bag can be used with a grease pencil to record the sample information, with minimum storage space, very safe, reliable, convenient, economic, and cost-saving.

Opening method of sterile sample bag

a. Tear along the notch of the side with the seal.

b. Both hands index finger and thumb are placed in the homogeneous bag/sampling bag on both sides of the seal.

c. Rub back and forth with force.

d. Open the bag by pinching both sides of the bag with the index finger and thumb.

Sterile bags for microbiology

For sterile laboratory instruments, if sterility is not guaranteed, it is considered the most serious non-conformance event. Therefore, aseptic packaging materials should be able to provide a suitable microbial barrier to provide the integrity of the sterile barrier system and product safety. Medical dialysis paper has a slightly larger pore size and poorer microbial barrier and is suitable for medical devices that will soon be reused and re-sterilized (autoclaved) in hospitals, with an expiration date of just a few days (long basically no more than six months). For medical devices with a nominal expiration date of several years, packaging materials with good microbial barrier properties or pure film sealing systems should be used.

A. Preparation

a. Tools for aseptic sampling of packaging

It is critical to have the right tools for taking aseptic sampling instruments for the product or process. Unless the proper collection tools are used, the integrity of the sample will be questioned, or even the sample will be meaningless. To avoid not having the proper sampling tools, it is recommended that an analytical checklist for aseptic sampling be created to collect sampling tools.

The sample container should be pre-marked before it initially enters the processing area, like the sample number, date of sampling, the person taking the sample, etc. These will make it easier to sample under different plant conditions. Additional sample numbers are generally formalized during sample collection and therefore do not need to be pre-marked. The operator's tools and facilities, such as overalls, hairnets, or sterilized and clean shoes and boots must be available to help prove that the collector has not contaminated the food product or sample.

b. In-line samples

Production line samples are generally raw materials, water used in the production of raw materials, packaging materials, or any other materials used in the production line. In-line samples are generally used to determine if the source of bacterial contamination is from the raw material or some part of the process.

c. Environmental samples

Environmental samples are taken with bacterial swabs, (bacterial swab samples do not give/measure timed results for microorganisms because the samples are very small and significant microorganisms are often lost). Cotton swabs are generally taken from food contact surfaces, and floor spills.

d. Other tools

a) Dry ice
Dry ice, also known as gel backs. Some types of refrigerants are necessary if the samples are to be kept cool during storage and transportation. Before using dry ice, the operator needs to check to see if there is contact with the dry ice bag, as it may contaminate the sample if it leaks. The operator may also use wet ice instead of dry ice. However, if the operator wants to keep the sample frozen, dry ice should be obtained before the test.
b) Boxes or coolers
Inspectors need to store and transport laboratory samples. If the samples do not need to be frozen, then a box can be used. However, if the samples need to be cooled, a standard refrigeration dish or holding tank is needed. Usually, a plastic bag is included with the refrigeration dish. The sample can be returned to the bag, while the refrigerant like dry ice can be placed outside the bag so that the possibility of sample contamination by ice is avoided.
c) Sterilized containers
From plastic bags to sterilized gallon lacquered buckets that can be used for products with sharp edges such as crab and shrimp.
d) Sampling tools
Sampling tools include teaspoons, corner spoons, pointed pliers, and beakers. The selection of tool type is generally determined by the product being sampled. The sterilization date of all sampling facilities and containers should be checked. The sterilization time should be indicated on the label and packaging of the instrument facility.
f) Sterile cotton swabs
Sterile cotton swabs are generally used for swabbing instrument facilities and plant environmental areas. There is generally a proper procedure for using cotton swabs. Step 1, open the cotton swab and peel off the skin, then it must be carefully placed on the test tube head, taking care not to stain the outer end of the swab; Step 2, wipe the area to be sampled, like the head of a case or the top tube; Step 3, carefully place the swab from the test tube head - pile it in until the middle of the tube.
g) Sterile sample bags
Sample bags must be sterile. When the operator is in use, simply tear off the closure, open the bag with the place provided, put the sample in, then roll the top of the bag and tie it firmly with a string; the bottom should be folded twice so that the string does not penetrate the plastic bag and cause the sample to leak. When samples are collected, the conditions at the time of sample collection (e.g., the temperature of the product, location, markings, etc.) need to be recorded in the inspector's notes. Samples taken can be distinguished from the sample number, date of collection, additional sample number, initial investigator, and other identifying information matter.

When collecting sterile samples, one of the most important rules is "never contaminate the sample". This requires the sample collector to take all additional samples very carefully, using sterile sample bags if necessary to ensure that this rule is not violated.

B. Microbiological aseptic sampling operation

a. All sampling utensils used for experiments must be sterilized (e.g., sample bags, samplers, blood collection tubes, test tubes, spatulas, spoons, knife-like tools, etc.).

b. Collect sample information. Label the container or sample bag containing the sample immediately before or after sampling, and each sample must be clearly labeled (e.g. name, source, quantity, location of sampling, sampler and date of sampling, etc.).

c. It is recommended to take 6 or more replicates per group, with at least 3 replicates.

d. Samples with sampling tubes can be transported at room temperature; if the tubes are not used, they must be stored at a low temperature of -80°C. Do not freeze and thaw repeatedly during storage; when sending at low temperature, please place the samples in a foam box and send them on dry ice.

Acquisition and acceptance of sterile sample bags

sampling-bag-sterile

Acquisition of sterile sample bags

From the point of view of the quality of the medium itself, there are differences between products of different manufacturers and even products of the same manufacturer with different batch numbers. When purchasing sterile sample bags, buyers should choose companies with a high reputation in the product market, quality assurance capability, and service guarantee capability. 

Acceptance of sterile sample bags

After the arrival of sterile sample bags, there should be a person to accept and accept the work. In acceptance, the person in charge should carefully check the information provided by the manufacturer, the name of the medium, number, appearance characteristics, batch number, shelf life is in line with the requirements.

How to buy sterile sample bag?

ANTITECK provide lab equipment, lab consumable, manufacturing equipment in life sciences sector.
If you are interested in our sterile sample bag or have any questions, please write an e-mail to [email protected], we will reply to you as soon as possible.


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