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The Science Behind 96 Deep Well Plates Materials

Posted on January 24, 2025 by Lydia96 Deep Well Plate Filling and Sealing Machine

Material selection plays a vital role in the performance of 96 deep well plates. Polypropylene and polystyrene are preferred due to their unique properties. Polypropylene resists acids, alkalis, and organic solvents, ensuring chemical stability during experiments. It also withstands autoclave sterilization at 121°C without losing its shape. These plates support workflows like sample storage, preparation, and high-throughput applications in genomics, proteomics, and clinical diagnostics. High-quality 96 deep well plates ensure durability and reliability, making them indispensable in laboratories.

Key Takeaways

  • Polypropylene is popular for 96 deep well plates. It resists chemicals and handles high heat, making it useful in labs.
  • Good deep well plates are strong and dependable. They lower mistakes and help labs work better.
  • Automation-ready plates work with robots. They speed up work and reduce human errors.
  • Plates with raised edges help stop mixing of samples. This keeps experiments accurate.
  • Always check for ANSI compliance and clean certifications. This ensures plates are high quality and protect samples.

Materials Used in 96 Deep Well Plates

Polypropylene

Polypropylene is the most commonly used material for manufacturing 96 deep well plates. Its affordability and versatility make it a preferred choice in laboratories. This material offers excellent chemical resistance, allowing it to withstand exposure to acids, alkalis, and organic solvents without degrading. These properties ensure that polypropylene plates maintain their integrity during chemical reactions, making them reliable for various applications.

Polypropylene also exhibits high thermal resistance. It can endure autoclave sterilization at temperatures up to 121°C without deforming. This feature ensures sterility and durability, even under extreme laboratory conditions. Additionally, its mechanical strength prevents cracking or warping, even when subjected to pressure or repeated use. These qualities make polypropylene ideal for thermostable 96 deep well plates used in high-throughput workflows.

PropertyDescription
Chemical ResistanceResists acids, alkalis, salts, and organic solvents, ensuring stability during experiments.
High Temperature ResistanceWithstands autoclaving up to 121°C, maintaining shape and sterility.
Mechanical StrengthPrevents cracking or deformation, ensuring long-term durability.

CAPP Expell product range includes deep well plates made from high-quality virgin polypropylene. These plates are designed for reliable performance across diverse laboratory applications, including sample storage and drug screening.

Polystyrene

Polystyrene is another material used in 96 deep well plates, though it is less common than polypropylene. It offers excellent optical clarity, making it suitable for applications requiring visual inspection or spectrophotometric analysis. However, polystyrene has significant limitations. It lacks the chemical resistance of polypropylene and is vulnerable to many solvents. This material also exhibits poor thermal stability, as it tends to decompose at high temperatures.

Despite these drawbacks, polystyrene remains useful for specialized applications where optical properties are critical. For instance, deep well plates with raised rim design made from polystyrene can help prevent cross-contamination during sample handling. However, laboratories must carefully consider its limitations when selecting this material for specific workflows.

Other Synthetic Polymers

In addition to polypropylene and polystyrene, other synthetic polymers are occasionally used in 96 deep well plates. These materials are selected for their unique properties, such as enhanced chemical stability or compatibility with automation systems. For example, some plates are designed to integrate seamlessly with high-throughput automation workflows, ensuring efficiency and precision.

High-quality polymers like those used in the capp expell product range offer a balance of chemical resistance, temperature tolerance, and mechanical strength. These versatile 96 deep well plates are suitable for demanding applications, including genomics, proteomics, and clinical diagnostics. By choosing the right material, laboratories can optimize performance and reliability in their experiments.

Properties of Chemically Stable Deep Well Plates

Chemical Stability

Chemically stable deep well plates play a crucial role in laboratory workflows. These plates, often made from polypropylene, exhibit exceptional resistance to a wide range of chemicals, including acids, alkalis, salts, and organic solvents. This chemical stability ensures that the plates maintain their structural integrity and do not interact with reaction components. By remaining inert, they safeguard sample integrity and prevent contamination during experiments.

Polypropylene's chemical resistance makes it ideal for handling highly corrosive liquids. It performs reliably in fields such as chemistry, biomedicine, and pharmaceuticals. Additionally, its ability to withstand repeated exposure to chemical reagents without degradation ensures consistent performance over time. Laboratories benefit from this durability, as it reduces the risk of experimental errors caused by material breakdown.

Tip: For workflows involving corrosive substances, polypropylene deep well plates provide a dependable solution due to their inert properties.

Thermal Resistance

Deep well plates must endure a wide temperature range to support diverse laboratory applications. Polypropylene plates excel in this regard, remaining stable at temperatures as low as -80°C for ultra-low temperature storage. They also withstand high-temperature autoclave sterilization up to 121°C without deforming. This thermal resistance ensures sterility and structural integrity, even under extreme conditions.

Temperature RangeDescription
-80°CStable for ultra-low temperature storage
121°CWithstands high-temperature autoclave sterilization

These properties make polypropylene plates suitable for workflows requiring both cryogenic storage and high-temperature disinfection. Their ability to maintain physical properties across this range enhances their versatility in laboratory settings.

Mechanical Strength

Mechanical strength is another critical property of deep well plates. Polypropylene plates resist cracking and deformation, even under significant external forces. This durability ensures reliable performance in high-load applications, such as high-throughput screening and sample storage. Their structural stability minimizes the risk of equipment damage during experiments, enhancing overall efficiency.

High mechanical strength also allows these plates to endure repeated use without compromising their functionality. This reliability makes them indispensable for laboratories that rely on automation and high-throughput workflows. By choosing plates with robust mechanical properties, researchers can ensure consistent results and reduce operational costs.

Note: The combination of chemical stability, thermal resistance, and mechanical strength makes polypropylene deep well plates a versatile choice for demanding laboratory applications.

Application-Specific Considerations for Automation-Ready Deep Well Plates

High-Throughput Automation

Automation-ready deep well plates are essential for laboratories utilizing high-throughput systems. These plates must meet specific requirements to ensure compatibility with automated liquid handling machines and robotic systems. Features such as dimensional precision and ANSI compliance guarantee seamless integration into automated workflows.

FeatureDescription
MaterialBest synthetic polymers, virgin polypropylene for reliable performance
Dimensional PrecisionEngineered for minimal variation between wells for consistent treatment conditions
ANSI ComplianceConforms to American National Standards Institute standards for quality validation
Automation-ReadyDesigned for compatibility with automated liquid handling machines (e.g., Hamilton Microlab® STAR)
Cross-Platform CompatibilityOpen design allows use in both manual and automated workflows

These plates also enhance laboratory throughput by reducing manual operations and minimizing errors. Their increased well depth accommodates larger sample volumes, making them suitable for workflows requiring high sample capacity.

Cross-Contamination Prevention

Cross-contamination poses a significant challenge in high-throughput modern laboratory environments. Automation-ready deep well plates address this issue through innovative design features.

  • CAPP 96 deep well plates use chemically stable polypropylene, which minimizes interactions with reaction components.
  • The material's inert properties prevent potential leakages during experiments.
  • Raised rim designs further reduce cross-contamination by creating a physical barrier between wells.

The raised rim design reduces cross-contamination of samples, which can be a huge problem when working with large sample numbers.

These features ensure sample integrity, even in workflows involving corrosive substances or sensitive reagents.

Specialized Applications

Automation-ready deep well plates support specialized applications requiring unique material properties. Polypropylene's resistance to solvents and durability under repeated chemical exposure make it ideal for processes like DNA/RNA extraction and protein analysis.

  • Sample pooling and aliquoting for analysis
  • Precipitation and centrifugation reactions
  • Solid-phase extraction (SPE) techniques
  • Sample concentration through evaporation or lyophilization

These plates also ensure reproducibility in experiments. Their contaminant-free design prevents interference from DNases, RNases, or endotoxins, safeguarding sample quality. Each well's capacity of 1 to 2 mL reduces evaporation risks, making them suitable for long-term storage and stability under low-temperature conditions.

Standards for High-Quality 96 Deep Well Plates

ANSI Compliance

ANSI compliance ensures that 96 deep well plates meet standardized dimensions and quality benchmarks. These plates conform to ANSI/SLAS footprint standards, which specify precise dimensions of 127.76mm ± 0.25mm in length and 85.48mm ± 0.25mm in width. This standardization guarantees compatibility with a wide range of laboratory equipment, including automated liquid handling systems and robotic platforms.

CAPP Expell deep well plates adhere to ANSI standards, making them a reliable choice for laboratories. This compliance simplifies the validation process for quality specifications, ensuring that the plates perform consistently across various workflows. Laboratories benefit from this uniformity, as it reduces the risk of errors caused by dimensional inconsistencies.

Tip: Always verify ANSI compliance when selecting deep well plates to ensure seamless integration with laboratory equipment.

Contaminant-Free Certification

Contaminant-free deep well plates are essential for maintaining sample integrity in sensitive applications. Certifications ensure that these plates are free from common molecular contaminants such as DNases, RNases, human DNA, and endotoxin inhibitors. For instance, CAPP 96 deep well plates undergo manufacturing in a class 100K clean room environment, ensuring a contaminant-free sample holding environment.

Product NameCertificationsDescription
VWR® 96-Well Deep Well PlatesMade in Class 10000 cleanroomExtractable-free polypropylene that will not leach unwanted compounds.
PurePlus® Well PlatesCertified free of RNase, DNase, and ATP; compliant with ISO 11137Available in sterile or non-sterile styles, lot tested.
CAPP 96 Deep Well PlatesManufactured in a class 100K clean roomCertified free of DNases, RNases, human DNA, and endotoxin inhibitors.

These certifications address significant concerns in life science experiments, where contaminants can compromise results. High-quality plates, such as those made from polypropylene, also offer excellent thermal resistance, allowing autoclave sterilization up to 121°C without deformation. This ensures sterility and structural stability during use.

Laboratories evaluate the quality of deep well plates by examining their certifications and manufacturing processes. Plates crafted in controlled environments, like class 100K clean rooms, meet international quality standards. Rigorous quality control measures ensure uniformity and reliability, making these plates indispensable for workflows requiring precision and safety.

Note: Contaminant-free certifications are critical for applications like DNA/RNA extraction, where even trace contaminants can affect experimental outcomes.

Conclusion

The choice of materials, such as polypropylene and polystyrene, plays a crucial role in the manufacturing of 96 deep well plates. High-quality materials like polypropylene ensure chemical stability and thermal resistance, which are essential for creating reliable and durable plates. These properties allow the plates to withstand extreme temperatures and corrosive substances, making them versatile for various laboratory applications and workflows.

Selecting the right 96 deep well plates for specific applications improves laboratory efficiency. Plates designed for automation reduce manual operations and minimize errors, while features like raised rims prevent cross-contamination. Certifications for contaminant-free manufacturing further ensure sample integrity. By considering material properties and certifications, laboratories can optimize results and maintain accuracy in experiments.

Tip: Always evaluate the material and design of 96 deep well plates to match the demands of your workflow.

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