What is mini centrifuge machine?
The definition of mini centrifuge machine
Mini centrifuge machine which is also called a micro centrifuge machine, the speed can reach more than 20,000 rpm. it is a small in size centrifugal machine to be used in microfiltration, rapid dumping of reagent from test tube wall or cover, and slow centrifugation of test tube or tube row. Its greatest characteristic is its small size so it is very convenient to carry or remove. Mini centrifuge machines have commonly used in laboratory blood analysis.
The operating principle of mini centrifuge machine
As a mini centrifuge machine is used in microfiltration, that means when the suspension liquid which contains fine particles is standing still, the suspended particles gradually sink due to gravity. The sinking speed depends on the weight of the particle. In the contrast, the particle density which is smaller than the liquid will float up. A centrifuge is to use the powerful centrifugal force generated by the high-speed rotation of the centrifuge rotor to speed up the sedimentation rate of particles in the liquid and separate the matter with different sedimentation coefficients and buoyancy density in the sample.
Application of mini centrifuge machine
Because the microcentrifuge machine is small and light-weight, there is more and more industry to use it. Therefore, the product application scope mainly has the following points:
-- Cell isolation
-- The serum is extracted from whole blood
-- General precipitation use
-- Microfiltration of HPLC
-- Low solubility drug text
-- PCR experiment was partitioned by centrifugation
-- Permeation of cells with chelates or hydrophobic substances
-- Cells were removed from the medium after fermentation.
-- Samples prepared for PCR reaction, gel electrophoresis, and enzyme reaction
Features of mini centrifuge machine
Mini centrifuge machine also has the name of a microcentrifuge machine. Therefore the biggest selling point of microcentrifuge is very small and exquisite. The rotor speed can reach more than 20000 rpm. The Angle swivel head used in a centrifuge is made of titanium alloy and aluminum alloy. The centrifugal tube is made of polyethylene hard plastic with a cover. In addition, the centrifuge is equipped with a vacuum system. The mini centrifuge also has a drive belt that is opening. It just has a protective cover on the motor end.
In general, using a microcentrifuge machine is very convenient to install or replace the rotor because you do not need tools. If you open the cover during the operation, the centrifugal machine will stop working immediately.
Type of mini centrifuge machine
Micro-plate mini centrifuge machine
A microplate mini centrifuge is an instantaneous centrifugal machine designed specifically for 96 well plates. Using a breathless motor, low noise, and maintenance-free. It is very convenient for the operator to separate from the lower hanging well droplets. Generally speaking, most microplate centrifuge machines on the market are very large and they will occupy a lot of laboratory space at present. However, the microplate mini centrifuge machine completely solves this problem because of its unique design and small size. And the functions like the regular microplate centrifuge. In addition, the microplate is vertically put into the centrifuge rotor through the top slot of the microcentrifuge. And there is no reason to worry about leaking because the liquid is kept at the bottom of the microplate. The speed can accelerate to 2500 rpm instantly when the rotor is working.
In a word, the microplate mini centrifuge machine has the advantages of fast centrifugal wall hanging droplets, use before PCR experiment, significantly improve the amplification effect, and is suitable for all kinds of standard PCR microwell plate with skirt edge or not.
Micro high speed centrifuge machine
The micro high-speed centrifuge is a practical, reliable laboratory assistant, it has humanized design and the rotor speed can up to 14,500 rpm. It uses a brushless maintenance-free high-speed motor. In addition, the noise is very low, the weight is very light and the service life is very long. The rotor can autoclave.
The micro-high speed centrifuge machine
can satisfy the routine need of universities, research institutions, foodstuff and medical and health institutions, and other industries.
Mini palm centrifuge machine
Mini palm centrifuge machine means that you can hold it with one hand. The shape of the mini palm centrifuge machine is compact and exquisite, it is also a one-click operation. This mini-type centrifuge machine is suitable for rapid micro centrifugation and microfiltration and is especially suitable for use as a personal centrifuge.
The rotors of mini centrifuge machine
There are two rotors can be used in a mini centrifuge machine. One is an angular rotor, the other is a PCR rotor
Angular rotor of mini centrifuge machine
Angular rotor can be placed 8×1.5ml tube, with standard casing can be used for 0.5ml and 0.2ml tube.
The PCR rotor can hold 16 0.2ml 8-line tubes, or 16 individual 0.2ml tubes.
Determinants of liquid phase clarity after mini centrifuge clarification
As we know, the function of mini centrifugal machine is separate the solid in the suspension from liquid or remove liquid from wet solid.
Therefore, there are two decisive factors for liquid phase clarity after mini centrifuge clarification. One is effective centrifugal force acting on the liquid phase in the sedimentation area of the drum. The other is the average residence time of liquid phase in the drum sedimentation area. And there are also two factors to influence the moisture content of solid phase after mini centrifuge clarification. One is the effective centrifugal force acting on the solid phase in the dehydration area of the drum, the other is the average residence time of the solid phase in the dehydration area of the drum.
So we can adjust the working state of micro centrifuge machine and control above factors within limits to obtain better separation effect. In a normal circumstance, the first thing is to set the drum speed range for which material to be separated. Then adjust the difference speed and spillplate according to the separation condition of micro centrifuge to obtain better separation effect.
It has some regulations about adjusting the differential speed and overflow plate and liquid phase clarity and solid phase humidity:
-- A small difference of rotation speed will reduce the moisture content of the solid phase. The higher the rotational speed, the higher the liquid phase clarity, but the higher the moisture content of the solid phase
-- The overflow plate with smaller inner diameter of micro centrifuge will improve the liquid phase clarity, but the moisture content of solid phase will increase. The larger inner diameter of the overflow plate will reduce the moisture content of the solid phase, but the liquid phase clarification will be reduced.
How to use mini centrifuge machine?
1. Samples should be balance. Due to the the centrifuge tube of mini centrifuge is very small, the balance error that the machine can withstand is very small too. So the concentrator bowl and sample should be balanced at the same time when we use it.
2. The mini centrifugal machine should always be in a horizontal position, because the mini centrifuge is very small in size and light in weight. It is easy to rock if the machine is not in a horizontal position. This can lead to not work properly, or even have an accident.
3. If there is something wrong happen during working, please turn off the power immediately and reported to the technical personnel of the relevant company for maintenance.
4. Check and repair the performance of the machine regularly.
Brands of min centrifuge machine
Thermo Fisher Scientific
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